Place the flask in a hot water bath for four hours. THE PSILOCYBIN PRODUCERS GUIDE by Adam Gottlieb 1976 EXTRACTION Crumble and pulverize the dried mycelial material and combine each 100 mg of this material with 10 ml of methanol. Shulgin GOTTLIEB Then, after a little interaction at the web sites, PF found this extraction tek taken from a book by Adam Gottlieb at the EROWID web site. As to the identity of the crystals that were drifting around in the cooled Soxhlet receiver, from their being insoluble in ethanol, and white, and transparent, I would guess that you are seeing pure psilocybin. Efficient extraction apparently requires patience. Using this information they determined that the levels of psilocybin and psilocin are substantially the same in Psilocybe bohemica, in conflict with the published literature values where very small amounts of psilocin were observed. They conclude that many of the low psilocin assays of mushrooms are due to this difficulty of getting the alkaloid out of the plant and into the extracting solvent. Psilocin is extracted at a much slower rate than is psilocybin because it is contained intracellularly in the plant, and thus slower to be gotten out. These researchers were apparently surprised by these findings, as they explored further and uncovered other clues. But methanol was extremely inefficient regardless of the amount of water present in it. With aqueous ethanol, the optimum extraction was with a 70% ethanol concentration, and the extraction efficiency dropped almost to zero when there was no water present. But the numbers with psilocin are strange. The compound is, after all, a perfect example of a zwitterion, the internal salt of a phosphoric acid and an amine base.
With both, the less water present, the better. And the extraction efficiency is optimum with methanol and almost as good with ethanol. It is less soluble in boiling methanol, and almost insoluble in boiling ethanol. This alkaloid is reasonably soluble in boiling water from which it can be nicely crystallized. The isolation of psilocybin seemed to be quite reasonable. The results were, to me, both unexpected and most provocative. These Czechoslovakian scientists studied the efficiency of both methanol and ethanol as solvents, each containing varying amounts of water. The citation to the article is Kysilka, R. Do you know what these crystals are? - Journeyman Dear Journeyman: There is a fascinating report in the literature that gives a quantitative measurement of the efficiency of extraction of both psilocybin and psilocin from the mushroom Psilocybe bohemica. When the 60 mL of extract cooled to room temperature, many small transparent, colorless crystals had formed on the bottom of the container and did not redissolve on agitation. Shulgin: A friend of mine performed a Soxhlet extraction of 12 grams of powdered Psilocybe cubensis, using 95% ethanol. The assembly is supported by a clamp screwed to a vertical rod, itself fixed to the heating mantle.This is not my work but it seems that if you follow some of these procedures and the filter the Psilocybin crystals once they pecipitate from solution you would have fairly pure Psilocybin here is the link THE CRYSTALS OF THE GODS (anonymous experimenter who followed the Professors 190 proof extraction TEK) Suckerfree (Suckerfree) Posted on Wednesday, Aug07:26 pm: MaPsilocybe Mushroom Extractions Dear Dr. Flexible plastic tubing connects the condenser to the water cooling unit. The first comprises a boiling flask, soxhlet extractor and condenser. The apparatus consists of a distillation/extraction glassware unit and a heating mantle with thermostatic controller. Due to the difference in boiling point temperatures, only the solvent evaporates while the oil finds itself trapped in the flask.
When the latter is full, the dirty solvent returns to the flask via a siphoning reflux sidearm located at the base of the sample chamber. The condensate falls back into the sample chamber.
The solvent in this closed system is externally heated and vaporized continuusly in a flask, flow through the samples in the extraction tube, condenses at the top of the assembly. The samples are placed in the extractor and cleaned by refluxing solvent. The cleanliness of the sample is determined from the color of the solvent once it has permeated from the samples and condensed. Soxhlet distillation extraction removes liquid phases (oil and brine) from core samples by virtue of a solvent vaporization and condensation process.
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